JASCO PU 2080 PLUS
Principle
HPLC (High Performance Liquid Chromatography) is considered as one of the most important separation techniques among all other techniques. It depends on interaction of analytes with the stationary phase and the mobile phase, based upon its affinity and thereby separation. Based upon mechanism for separation, HPLC follows any one of the following mechanism, viz. adsorption, partition, ion exchange, ion pair or size exclusion mechanism.
Application
HPLC technique is generally used for separation, identification, purification and quantification of a compound from a mixture. The other major applications of HPLC include identification of percentage purity of APIs and separation of impurities; to control drug stability; tablet dissolution study; pharmaceutical quality control; stability studies, etc.
HPLC is useful to Pharmaceutical and Chemical industries to check purity of APIs/compounds and to find out presence of impurities. It is also useful in the food industry for quality control and to compare with standards, controlled by government.